CUSTOM ITEM: HPLC Column, RP PFP, 3um, 4.0mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
68322-25P-4 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it especially effective for halogenated, aromatic, and polar analytes. It also serves as a suitable alternate column for USP methods where PFP selectivity is beneficial.
With a 3 µm particle size, this column delivers greater efficiency than 5 µm columns, producing sharper peaks and improved resolution. As expected, the smaller particle size results in increased backpressure compared to larger particle columns.
250 mm Length – Maximum Resolution for the Most Demanding Separations
The 250 mm length provides the highest resolving power available in this format, making it ideal for complex samples and difficult separations where maximum peak capacity is required.
Maximum resolution compared to shorter column lengths
Superior separation of closely related and co-eluting compounds
Highest peak capacity for complex matrices
✅ Recommended for method development, impurity profiling, and highly complex analytical workflows
Flow Rate & System Requirements (4.0 mm ID, 3 µm)
⚠️ Compatible with standard analytical HPLC systems, with higher backpressure due to length and particle size
Typical flow rate range: ~0.7 – 1.2 mL/min
Higher efficiency than 5 µm columns
Increased backpressure from both smaller particles and longer column length
Suitable for UV and standard analytical detectors
✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection to maintain column performance
Why Choose PFP (F5) Columns for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with fluorinated and halogenated analytes
Improved selectivity vs C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility for method development
Versatile 100 Å Pore Structure
Suitable for small to moderately sized molecules
Balanced retention and performance
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry
High Efficiency (3 µm Particles)
More efficient than 5 µm columns
Sharper peaks and improved resolution
High Purity Silica
Consistent and reproducible chromatographic performance
Long column lifetime
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Polar compound retention
Pharmaceutical QA/QC workflows
Method development and impurity profiling
USP alternate methods
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Designed for standard analytical HPLC systems (4.0 mm ID)
Higher backpressure due to 3 µm particles and 250 mm length
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable